Expression in Progeny Cells Mosaicism and Heterogeneous Gene Integration and Results in Transduction Compromised by a Delay in Proviral Hematopoietic Progenitor Cells Is Lentivirus Gene Transfer in Murine
نویسندگان
چکیده
10.1128/JVI.74.24.11911-11918.2000. 2000, 74(24):11911. DOI: J. Virol. Didier Trono and Stefan Karlsson Hildur Helgadottir, Isao Hamaguchi, Sten-Eirik Jacobsen, Hanna Mikkola, Niels-Bjarne Woods, Marketa Sjögren, Expression in Progeny Cells Mosaicism and Heterogeneous Gene Integration and Results in Transduction Compromised by a Delay in Proviral Hematopoietic Progenitor Cells Is Lentivirus Gene Transfer in Murine
منابع مشابه
Lentivirus gene transfer in murine hematopoietic progenitor cells is compromised by a delay in proviral integration and results in transduction mosaicism and heterogeneous gene expression in progeny cells.
Human immunodeficiency virus type 1-based lentivirus vectors containing the green fluorescent protein (GFP) gene were used to transduce murine Lin(-) c-kit(+) Sca1(+) primitive hematopoietic progenitor cells. Following transduction, the cells were plated into hematopoietic progenitor cell assays in methylcellulose and the colonies were scored for GFP positivity. After incubation for 20 h, lenti...
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